We offer a broad range of genetic modifications of mice using CRISPR/Cas9
This include but is not limited to:
- Generation of indel strains for any particular gene in F1 hybrid (C57BL/6 x CBA) or pure C57BL/6 background. With this approach targeting properly designed site can generate constitutive knockouts, inactivate a particular splicing isoform, or remove an important regulatory sequence in the genome.
- Generation of deletions at any locus of the mouse genome in F1 hybrid (C57BL/6 x CBA) or pure C57BL/6 background. This approach will utilize two gRNAs and can be used to remove entire exons or regulatory sequences in non-conditional manner.
- Generation of knock-in mice in F1 hybrid (C57BL/6 x CBA):
- Insertion N- or C-terminal (FLAG, EGFP, HA, etc.) tags in locus, which results in expression of a tagged protein from the endogenous gene.
- Construction of a mouse line expressing Cre recombinase used for conditional KO in specific tissues, either by targeting the ROSA26 locus with Cre recombinase genes accompanied by a specific promoter or by insetting Cre after a tissue specific gene with an IRES of 2A autocleavable sequence.
- Introduction of specific point mutations: disease related mutations, catalytic mutations etc.
- Generation of specific Cre regulated conditional regulated alleles such as COIN methodology, where the conditional gene trap module (COIN module) is inserted into the target gene in an orientation opposite to the gene’s direction of transcription. Activation of Cre recombinase causes inversion of the COIN module, which results in termination of transcription and expression of a reporter, thereby inactivating the target gene and conveniently marking the cells where the inversion occurred.